Identification of Genes that Elicit Disuse Muscle Atrophy via the Transcription Factors p50 and Bcl-3
Figure 1
Quantitative real time PCR (qPCR) to measure mRNA expression of selected atrophy genes.
Taqman probe and primer sets were used to confirm mRNA expression from microarray data of gastrocnemius and plantaris muscles. All 14 genes measured confirmed a significant upregulation due to 6 days unloading (HU) compared to weight bearing (WB) in muscles of wild type (WT) mice. Gene expression due to unloading in knockout mice (Nfkb1 and Bcl-3) was either not different from weight bearing or it had a significantly less increase for all cases except Fbxo9 and Foxo3 in Nfkb1−/− mice. Gene expression was measured for: A) Trim63 (MuRF1), B) Fbxo32 (MAFbx), C) ubiquitin C, D) Fbxo9, E) Psma6, F) Psmc4, G) Psmg4, H) Ctsl, I) Foxo3, J) Runx1, K) Ankrd1 (Carp), L) Tnfrsf12a (Tweak receptor), M) Eif4ebp1, N) Cxcl10 (IP-10), and O) GAPDH. *significantly different from weight bearing value (P<0.05), †significantly different from wild type unloaded value (P<0.05).