Insulin Signaling, Lifespan and Stress Resistance Are Modulated by Metabotropic GABA Receptors on Insulin Producing Cells in the Brain of Drosophila
Figure 4
GABAB receptor knockdown on insulin-producing cells affects DILP levels.
A. Relative DILP immunofluorescence in IPCs in fed and starved flies with and without GABAB receptor knockdown (GBRi) in IPCs (Dilp2-Gal4/UAS-gbr2-RNAi). The DILP antiserum used is likely to cross react with DILP2, 3 and 5 [16]. Control flies (GBRi-w1118) display significantly lower levels of DILP-immunofluorescence than the flies with GBRi (Dilp2-GBRi), both in fed flies (p<0.001; Anova with Tukey's comparison) and after starvation (p<0.001). A smaller, but significant, increase in DILP fluorescence is seen at starvation for both genotypes (p<0.05 in both cases). 19–35 cells were measured in 5 specimens of each genotype. B–E. representative confocal images of DILP-immunolabeled IPC of the fed and starved control (GBRi-w1118) and experimental (Dilp2-GBRi) flies.