Functional and Pathogenic Differences of Th1 and Th17 Cells in Experimental Autoimmune Encephalomyelitis
Figure 8
Cytotoxic potential of Th1 and Th17 cells towards FT7.1 cells.
2D2 MOG-specific T cells were polarized in Th1 and Th17 conditions and co-cultured in duplicates with FT7.1 cells as described in methods. A. MHC class II expression on FT7.1 cells was analysed by FACS. B. GFP-labeled FT7.1 cells were co-cultured with Th1 or Th17 cells in the presence of isotype control or anti-MHC class II antibodies. Cells were tracked every 30 minutes by fluorescent time-lapse microscopy. Shown is the snap-shot fluorescent picture after 48 h co-culture. Magnification: 10×. C. Quantification of the fluorescent area (surviving cells) every 6 hours in the conditions shown in B. The values were normalized to that of control (FT7.1 cells only). D. IL-17 and IFN-γ levels were measured in the supernatants at 48 h after the co-culture by ELISA. Data shown are representative of minimum 3 experiments. ns-not significant.