Cationic Amino Acid Transporters and Salmonella Typhimurium ArgT Collectively Regulate Arginine Availability towards Intracellular Salmonella Growth
Figure 6
The ΔargT strain infection in the BMDMs gives rise to high NO response.
(A) BMDMs were infected at 10 MOI with the WT, ΔargT or the argT complemented strain (c- argT) and also with 30 MOI of the ΔargT strain and the argT complemented strain (c- argT) strain. Production of nitrite was determined in the culture supernatants of the infected BMDMs by Griess reaction after 12 h of infection. (B) BMDMs were maintained in 0.5, 1.0, 1.5 or 2.0 mM concentration of L-arginine and infected with the WT Salmonella for 12 h. Production of nitrite was determined in the culture supernatants by Griess reaction. Values are expressed as mean ±SD of one of three independent experiments performed in triplicate. Statistical significance was defined as follows: * P<0.05 (Student's t test).