De Novo Designed Proteins from a Library of Artificial Sequences Function in Escherichia Coli and Enable Cell Growth
Figure 4
Growth of auxotrophic strains of E. coli in selective liquid media.
In each panel, the bottom curve shows that the negative control expressing LacZ (open circles) does not support growth. In contrast, most of the positive controls (black circles) expressing the natural E. coli proteins grow well. (Fes is an exception because overexpression of E. coli Fes is toxic.) The designed proteins enable growth that is well above background. (A) ΔserB: LacZ, open circles; SerB, closed circles; Syn-SerB-1, closed triangles; Syn-SerB-2, open triangles; Syn-SerB-3, closed squares; and Syn-SerB-4, open squares. (B) ΔgltA: LacZ, open circles; GltA, closed circles; and Syn-GltA-1, closed triangles. (C) ΔilvA: LacZ, open circles; IlvA, closed circles; Syn-IlvA-1, closed triangles; and Syn-IlvA-2, open triangles. (D) Δfes: LacZ, open circles; Fes, closed circles; Syn-Fes-1, closed triangles; Syn-Fes-2, open triangles; Syn-Fes-3, closed squares; Syn-Fes-4, open squares; Syn-Fes-5, closed diamonds; Syn-Fes-6, open diamonds; Syn-Fes-7, ×; and Syn-Fes-8, +.