Discriminating Multi-Species Populations in Biofilms with Peptide Nucleic Acid Fluorescence In Situ Hybridization (PNA FISH)
Figure 7
Tri-species biofilm formation.
(A) Biofilm populations for 24 and 48 hours on each support material. (B) CLSM images distinguishing each bacteria and the superposition of the three fields. (D) CLSM showing the biofilm three-dimensional spatial distribution. A frontal quadrant (red rectangle) was removed to show the existence of an upper layer exclusively formed by E. coli, over a mixed Salmonella and Listeria layer. The bottom blank rectangle shows a transversal biofilm image showing the well defined layers.