Hydrogen Peroxide Probes Directed to Different Cellular Compartments
Figure 4
Changes in HyPer redox state following redox perturbations.
(A) Response of HyPer expressed in various compartments of NIH 3T3 cells to PGDF. NIH 3T3 cells transiently expressing HyPer were stimulated with 20 nM PDGF for 10 min. Images obtained before and after treatment are shown. (B) Dynamics of intracellular fluorescent HyPer response in NIH 3T3 cells treated with 20 nM PDGF or 50 µM H2O2. Graphs represent mean fluorescence intensity of one representative cell in 5 min time-lapse. (C) Generation of H2O2 during TNF-α signaling in various compartments of HEK 293 cells. HEK 293 cells were incubated with 40 ng/ml TNF-α for 6 h. Fluorescence was recorded at emission 530. (D) Inhibition of Complex I of mitochondrial ETC leads to H2O2 generation in HEK 293 cells. Data represent mean of 3 measurements, ± standard deviation.