Hydrogen Peroxide Probes Directed to Different Cellular Compartments
Figure 1
Response of HyPer expressed in HEK 293 cells to H2O2.
(A) Fluorescence excitation spectra were recorded from HEK 293 cells expressing HyPer 3 min after indicated concentrations of H2O2 were added to cells or 30 min after DTT treatment. Untransfected cells were used as a negative control. Emission was recorded at 530 nm. (B) Sensitivity of various peroxide sensors to H2O2 treatment. Emission was recorded at 530 nm for HyPer and DCF and at 510 nm for PG1. (C) Probing H2O2 with various fluorescent sensors in HEK 293 cells oxidized with H2O2 (5 min) or reduced with DTT (30 min). All data represent mean of 3 measurements ± standard deviation.