Epithelial Cell Transforming Sequence 2 in Human Oral Cancer
Figure 3
Evaluation of ECT2 protein expression in primary OSCCs.
(A, B) Representative IHC results of ECT2 in normal oral tissue and primary OSCC. (A) Normal oral tissue has no ECT2 protein expression. Original magnification, ×100. Scale bars, 50 µm. (B) ECT2-positive cases of OSCC. Positive immunoreaction for ECT2 is detected in the nucleus and cytoplasm. Original magnification, ×400. Scale bars, 10 µm. (C) State of ECT2 protein expression in nomal oral tissue and primary OSCC. To investigate protein expression of ECT2 in primary OSCCs, we carried out IHC. The ECT2 IHC scores are calculated as follows: IHC score = 1×(number of weak stained cells in the field)+2×(number of moderately stained cells in the field)+3×(number of intensely stained cells in the field). The ECT2 IHC scores for OSCCs and normal oral tissues range from 55.67 to 211.33 (median, 163.33) and 8.33 to 85.33 (median, 44.00), respectively. The ECT2 protein expression level in OSCCs is significantly higher than that in normal oral tissues (p<0.001; Mann-Whitney U test).