Defects in Innate Immunity Render Breast Cancer Initiating Cells Permissive to Oncolytic Adenovirus
Figure 4
Mislocalization of TLR9 in JIMT-1 CD44+/CD24−/low CIC breast cancer cells.
In contrast to normal tissue stem cells and ArLa non-CIC cancer cells, TLR9 (red) shows mislocalization in JIMT-1 CD44+/CD24−/low CIC population indicated by distinct distribution form the endosomal marker EEA1 (green) and partial co-localization with an ER-marker calnexin (ER, green) in both infected or non-infected cells, nuclear stain DAPI in blue (A). (B and C) Staining of adenovirus hexon (red) at 0 min, 30 min, 1 h and 4 h time points following infection, shows initial localization of the virus on cell surfaces and at later time points at 30 min up to 4 h internalization in endosomes, showing that Ad5/3-Delta24 is able to infect this cell type. DAPI nuclear staining in white (B and C). In infected JIMT-1 CD44+/CD24−/low CIC TLR9 (blue) is retained in the ER-golgi like structures (B, green) and does not traffic to the endosomes (C, green) to colocalize with the virus at time points up to 4 h post infection (B). Scale bars:10 µm.