Two-Photon Imaging of Calcium in Virally Transfected Striate Cortical Neurons of Behaving Monkey
Figure 8
Functional TPSM of single Neuron 3 (M4R, 14 days post injection, 300 µm depth).
(A) Low magnification structural image (200×200 µm) with the ROI (white rectangle). (B) High magnification structural image (100×100 µm) with labeled neuron in center. (A,B) YFP structural images (10 frames averaged). (C) FRET signal plotted as a function of time for all 8 directions (blue and red curves). Conventions otherwise as Figure 6A–6C. (D) Circular tuning curve (mean and standard error).