Two-Photon Imaging of Calcium in Virally Transfected Striate Cortical Neurons of Behaving Monkey
Figure 6
Functional TPSM of single Neuron 1 (M3R, 311 days post injection, 260 µm depth).
(A) Low magnification structural image (200×200 µm) with ROI (white rectangle) submitted to further analysis. (B) High magnification structural image (50×50 µm) with brightly labeled neuron in center. (A,B) YFP images (10 frames averaged). (C) FRET signal plotted as a function of time for all 8 directions (blue and red curves). First panel also shows blank signal (gray curve), onset of fixation (dashed line), and 500 ms baseline signal (gray shaded rectangle) used for normalization (stimulus onset was at 0 ms). (D) Circular tuning curve (mean and standard error).