Versican G3 Promotes Mouse Mammary Tumor Cell Growth, Migration, and Metastasis by Influencing EGF Receptor Signaling
Figure 10
Versican G3 domain modulates breast cancer cell growth and migration via its epidermal growth factor-like motifs.
(a) Vector-, G3- and G3ΔEGF- transiently transfected 66c14 cells and 4T07 (2×104) were inoculated in 6-well culture dishes containing 10% FBS/DMEM and cultured for 3 days. Cells were counted under light microscopy. (b) Vector-, G3- and G3ΔEGF- transiently transfected 66c14 cells (2×105) were inoculated and cultured in 6-well culture dishes containing 10% FBS/DMEM for 12 h. all the samples were wounded by a sterile pipette tip to create a 1-mm cell-free path, washed with PBS, cultured in 10% FBS/DMEM medium with 2 mM hydroxyurea for 3 d. (c) The distances between the wounding centre and the front of the migrating cells (vertical axis) were measured for statistical analysis. (All groups compared with vector control cells, n = 10, * p<0.05, ** p<0.01, analyzed with t-test). (d) Cell lysates of vector-, G3- and G3ΔEGF- transiently transfected 66c14 cells were prepared and subjected to immunoblotting with antibodies to 4B6, pEGFR, EGFR, pERK and ERK2.