TLR 9 Activation in Dendritic Cells Enhances Salmonella Killing and Antigen Presentation via Involvement of the Reactive Oxygen Species
Figure 4
ROS inhibition by PAO rescued the Salmonella proliferation defect in the CpG DNA treated DCs.
DCs were pretreated for 30 min with or without PAO (5 µM, phenylarsine oxide) and then infected with the WT Salmonella with or without 10 µg/ml CpG DNA. After 2 h (open bar) and 16 h (filled bar) of infection the intracellular CFU was determined for both time points, and the rates of intracellular replication were expressed as CFU/ml. Experiments were performed in triplicate on two different occasions and the standard errors from the means are shown. Statistical significance was defined as follows: * P<0.05. (Student's t test).