Analysis of Thyroid Response Element Activity during Retinal Development
Figure 8
Assay of the 2xDR4 reporter in the developing chick retina.
A–D. 2xDR4 + pβactin-RFP/GAPDH-shRNA in varying T3 concentrations. 2xDR4 + GAPDH-shRNA -T3 (A, A'), 100 pM T3 (B, B'), 1 nM T3 (C, C'), and 100 nM T3 (D, D'). Closed arrow heads mark AP-negative patch. E–F. Cryosectioning of retina. 20 um cryosection of 2xDR4 + GAPDH-shRNA -T3 (E, E') and 100 nM T3 (F, F'). G–H. TRα and TRβ shRNA in 100 nM T3. 2xDR4 in 100 nM T3 + TRα-shRNA (G, G') or TRβ-shRNA (H, H').