Expression of GABAergic Receptors in Mouse Taste Receptor Cells
Figure 10
Mouse GAT4 immunoreactivity in circumvallate papillae from GAD67-GFP mice.
A Z-stack of 5 LSCMs (0.5 µm each, collected 1 µm apart) of mouse circumvallate taste buds with GFP expression identifying the GAD67-expressing taste cells is shown in panel A. The corresponding image of the taste buds labeled with an anti-rat GAT3 (mouse GAT4) antibody is shown in B with the DIC image shown in C. D, The overlay of A, B, and C reveals that most GAT3 labeling is localized in the surrounding cells near the basolateral portion of the taste bud. A few GFP expressing taste cells have some overlap with the mouse GAT4 immunoreactivity (see arrowheads). The negative control is the same as Figure 9. Scale bars = 20 µm.