ATM Modulates the Loading of Recombination Proteins onto a Chromosomal Translocation Breakpoint Hotspot
Figure 2
Focus formation and expression of RAD51 in ATM deficient cells.
(A) Immunoblotting analysis of BV173, AT5BIVA and 11-4 cells using an anti-RAD51 antibody. Cells were treated with etoposide (w30) or DMSO (Con) for 10 min and cultured for 30 min in normal medium. β-actin was used as a loading control. (B) The percentage of RAD51 foci positive AT5BIVA, 11-4 and BV173 cells was significantly increased after etoposide or bleomycin treatment (p<0.001, as determined by the Z test of homogeneity for independent samples). Values represent the means ± SE from three to four independent experiments (n>200 cells for each). (C) Focus formation of RAD51 in AT5BIVA and 11-4 cells after etoposide exposure. RAD51 nuclear foci were detected by immunofluorescence staining using anti-RAD51 antibodies. Scale bar: 5 µm.