Clostridium perfringens Epsilon Toxin Targets Granule Cells in the Mouse Cerebellum and Stimulates Glutamate Release
Figure 4
ET stimulates excitatory and inhibitory synaptic transmission onto the Purkinje cells.
A, right: spontaneous PSC detected in voltage-clamped Purkinje cells maintained at −60 mV, in absence (Cont) or 5 min after ET (10−7 M) application. The relative mean frequencies (Freq) and amplitudes (Ampl) of spontaneous EPSC (upper graph) or IPSC (lower graph), before (white bar) or 5–7 min after 10−7 M ET was added (black bar), n = 15 distinct experiments. B–D, same kind of measurements but after pre-treatment (B) with TTX (10−6 M for 10 min, n = 18, (C) with bicuculline (10−5 M for 5 min) to block the IPSC (n = 17), or (D) CNQX (10−5 M for 5 min) to block the EPSC (n = 18). The frequencies and amplitudes are presented as percent of control condition (i.e. without any treatment, white bars) or after pre-treatment (grey bars), and after subsequent application of ET (black bars). **: p<0.01, *: p<0.05, otherwise n.s. Same scale for all current traces.