Inhibition of Melanoma Growth by Subcutaneous Administration of hTERTC27 Viral Cocktail in C57BL/6 Mice
Figure 3
NK cell activity is crucial for the anti-tumor effect of hTERTC27.
PMBC from mice intraperitoneally injected with anti-GM1 antibody were analyzed for populations of NK cells (A), helper T cell/cytotoxic T cell/B cell (B) and regulatory T cell (D) by flow cytometry. (C) Cytotoxicity of NK cells against YAC-1. Lymphocytes isolated from spleen of each mouse were cultured with YAC-1 cells at various E/T ratios, and the specific lysis was determined using CytoTox 96 Non-Radioactive Cytotoxicity Assay Kit. (E) NK cell depletion abrogated the anti-tumor effects of rAAV-/rAdv-hTERTC27 in C57BL/6 mice. *: P<0.05, compared with EGFP group. (F) A representative photo of tumor morphology from different treatment groups. hTERTC27: hTERTC27 viral cocktail; EGFP: EGFP viral cocktail. *: P<0.05, compared with hTERTC27 group.