Regulation of Heparin-Binding EGF-Like Growth Factor by miR-212 and Acquired Cetuximab-Resistance in Head and Neck Squamous Cell Carcinoma
Figure 5
MicroRNA-212 regulates HB-EGF expression in cetuximab resistant cells.
A) Pro-HB-EGF expression following the transfection with miR-212 mimics in three cetuximab resistant cell lines. B) Growth inhibition rate of 1Cc8 cells with exogenous miR-212 mimics, cetuximab or a combination of miR-212 mimics and cetuximab measured by colony formation assay. C) MicroRNA-212 directly regulates HB-EGF by binding to the 3′ untranslated region of HB-EGF in cetuximab resistant 1Cc8 cells while it was not significant in cetuximab sensitive SCC1 cells (*, p = 0.47; **, p = 0.038). RLU – Raw Light Units. D) Growth rate comparison of SCC1 cells in the presence of cetuximab, exogenous miR-212 inhibitor, or a combination of miR-212 inhibitor and cetuximab measured by colony formation assay.