Dynamic Analysis of Vascular Morphogenesis Using Transgenic Quail Embryos
Figure 5
Different behaviors of dorsal and ventral walls of the forming dorsal aortae.
(a) The 2P image data (see Videos S9 and S10) was subjected to cell tracking analysis. A total 1,218 EC tracks established were subdivided into dorsal and ventral halves of the dorsal aorta walls based on the first H2B-eYFP signal emergence position. The number of cells tracked in the dorsal and in the ventral walls was 444 and 874, respectively (a–c, and also see Videos S9 and S10). The dorsal wall ECs arise inside of the dorsal aortae and move diverse directions (a). The ventral wall ECs arise from the lateral side of the dorsal aortae and move medially in a unidirectional manner (b). 3D-reconstruction also reveals that these cells have different origins and behaviors (lower panels). The ventral dorsal aorta wall ECs, which are migrating from lateral mesoderm, curve toward dorsal and subsequently intercalate with the dorsal wall ECs at the boundary. In contrast to the ventral wall cells, the dorsal wall ECs do not move across the dorso-ventral boundary. These EC behaviors are illustrated in (d); upper panel is a ventral angle view, lower panel is transverse views).