IL-2 Mediates CD4+ T Cell Help in the Breakdown of Memory-Like CD8+ T Cell Tolerance under Lymphopenic Conditions
Figure 3
Anti-IL-2 treatment inhibits migration of Clone 4 CD8+ T cells to the pancreas of InsHA mice.
Irradiated InsHA injected with CFSE-labeled naive Clone 4 Thy1.1+ CD8+ T cells plus HNT Thy1.1+ CD4+ T cells were treated with either a combination of anti-IL-2 mAbs (S4B6+ JES6-1) or an isotype matched control antibody. Mice were sacrificed on day 12 after transfer and the presence of donor T cells in different organs was analyzed. Data from one representative experiment out of four, including an average of 3 mice per group, are presented. (A) Levels of CFSE fluorescence on gated CD8+ Thy1.1+ donor lymphocytes in LN and pLN are shown. The mean percentages (± SD, n = 3) of highly proliferating CD8+ donor T cells are indicated. (B) Absolute numbers of CD8+ donor T cells in the lymphoid organs were enumerated following the treatment with anti-IL-2 mAbs (S4B6+ JES6-1) (black bars) and an isotype matched control antibody (empty bars). Results are presented as means ± SD (n = 3). (C) The presence of CD8+ Thy1.1+ donor T cells in the pancreas was evaluated by FACS. Numbers indicate FACS event counts in the depicted gates. Graphs represent mean (± SD, n = 3) recovered CD8+ donor T cells in control mice (empty bars), anti-IL-2 treated mice containing >103 donor cells (black bars) and anti-IL-2 treated mice containing <103 donor cells (grey bars).