Nucleo-Cytoplasmic Localization Domains Regulate Krüppel-Like Factor 6 (KLF6) Protein Stability and Tumor Suppressor Function
Figure 4
Identification of a Crm1-dependent KLF6/KLF6-SV1 nuclear export signal.
A, Hela cells transfected with EGFP-KLF6, EGFP-KLF6-SV1 or empty vector were treated with or without LMB for 2 h. B, The subcellular localization of truncated KLF6 constructs is shown. Cherry-H2A construct was used to show nuclear staining. Localization of the different constructs was observed by fluorescence microscopy. Graphs with the percentage of cells with the different localization are shown on the right. N, Nuclear localization, C, Cytoplasmic localization, N = C, Nuclear and cytoplasmic distribution within the same cell is equal, N>C, Nuclear localization is more intense than cytoplasmic localization, N<C, Nuclear localization is less intense than cytoplasmic localization, and PN, perinuclear localization.