Lethality and Developmental Delay in Drosophila melanogaster Larvae after Ingestion of Selected Pseudomonas fluorescens Strains
Figure 7
Population sizes of P. fluorescens in larvae of D. melanogaster.
Second instar larvae were inoculated with strains Pf0-1 (blue), SBW25 (green), or Pf-5 (red) (A and B), or the Pf-5 gacA mutant (open circle) or wildtype Pf-5 (filled circle) (C and D). Each day following inoculation, larvae were individually surface sterilized, homogenized and bacterial numbers were estimated by culturing. Each point represents the average of five larvae. Bacterial population sizes in the inocula were 104 cfu/plate (A and C), or 107 cfu/plate (B and D). The dotted line denotes the limit of detection. On Day 4, larvae inoculated with Pf0-1 at 107 cfu/plate had pupariated and were not sampled. Error bars denote standard errors.