A Novel Zinc Finger Protein Zfp277 Mediates Transcriptional Repression of the Ink4a/Arf Locus through Polycomb Repressive Complex 1
Figure 4
Zfp277 is required for the re-localization of PRC1, but not PRC2.
A. A schematic representation of the Ink4a/Arf locus in MEFs. ChIP assays were performed using anti-Bmi1, Ring1B, Mel18, H2AK119ub1, Ezh2, Jmjd3, and H3K27me3 antibodies. Regions amplified from the precipitated DNA by site-specific quantitative PCR are indicated by arrows. B–H. Q-ChIP analysis of the p19Arf exon 1b and p16Ink4a exon 1a. Wild-type MEFs and Zfp277−/− MEFs at passage 2 with or without NAC treatment were subjected to ChIP analyses using the indicated antibodies. Percentages of input DNA are shown as the mean ± S.D. for multiple experiments (n>6). Statistical significance was determined with Student's t-test; **p<0.01, ***p<0.001.