Thyroid Hormone May Regulate mRNA Abundance in Liver by Acting on MicroRNAs
Figure 4
Identification of the target genes of miR-206 that are regulated by TH.
A. Levels of miR-206 in AML 12 cells stably transfected to ectopically express miR-206. AML12 cells were transfected with pEGP-mmu-mir-206 expression vector or pEGP-mir null control vector and selected with puromycin and green florescence protein by microscopy. The expression of miR-206 was examined with the Taqman miRNA Assay (n = 5). B. The expression of genes that are putative targets of miR-206 in the two cell types shown in figure 4A was examined with RT-PCR (n = 3). C. The effects of TH on the expression of miR-206 in the transfected cells. The miR-206 transfected cells were treated with 10 nm T3 for 24 hours. MiR-206 expression was analyzed with Taqman miRNA Assay (n = 3). D. The effect of TH on the expression of the miR-206 target genes in the transfected cells. The miR-206 transfected cells were treated with 10 nm T3 for 24 hours. The expression of target genes was examined with RT-PCR (n = 3). Data are presented as mean ± SE. A two-tailed Student's t-test was used to calculate significance. * indicates p<0.05.