Thyroid Hormone May Regulate mRNA Abundance in Liver by Acting on MicroRNAs
Figure 2
Expression of miRs-1, 206, 133a and 133b in other animal models with altered TH levels.
A. Hepatic miRNA expression in livers of PND 15 mouse pups rendered hypothyroid by treatment with drinking water containing 0.04% (wt/vol) of PTU from GD 13 to PND 15. RT-PCR was performed with the Taqman miRNA Assay with RNA derived from male pups (3 per group). B. Hepatic miRNA expression in livers of PND 15 mice whose TH levels were modulated as follows: hyperthyroid pups (hyper) received a s.c. injection of T4+T3 (50 µg+5 µg, respectively per 100g bw) four hours prior to sacrifice and corrected hypothyroid pups received drinking water containing MMI and perchlorate (0.05 and 1% wt/vol, respectively) from PND 12 to 15 and an injection of T4+T3 (20 µg+2 µg, respectively per 100g bw) four hours prior to sacrifice, while control mice received an injection of PBS only. RT-PCR was performed with the Taqman miRNA Assay with RNA derived from male pups (3 per group). Data are presented as mean ± SE (n = 3). A two-tailed Student's t-test was used to calculate significance. * indicates p<0.05.