Cross-Reactivity of Herpesvirus-Specific CD8 T Cell Lines Toward Allogeneic Class I MHC Molecules
Figure 4
Allorecognition of human endothelial cell cultures by CD8 T cell lines enriched in herpesvirus-specific T cells.
T cell lines from D01 and D03, sorted with pp65495–503/A*0201 multimers, were tested against HAEC #4373, #3315, and #3376, while T cell line from D15, sorted with BZLF154–64/B*3501 multimers, was tested against HAEC #3643 (Cw*0602+) and #1415 (Cw*0602-) for TNF-α production (A) and cytotoxicity (Effector-Target ratio 15∶1) (B). TNF-α production was measured as indicated in Figure 2 legend. Only the endothelial cell lines expressing the relevant allogeneic HLA allele induced cytotoxicity and TNF production by the CD8 T cell lines tested. The data are representative of 3 different experiments.