TOR Is a Negative Regulator of Autophagy in Arabidopsis thaliana
Figure 2
RNAi-AtTOR plants have constitutive autophagy.
A Seven-day-old WT and RNAi-1 to RNAi-5 seedlings were stained with MDC and observed by fluorescence microscopy. Arrows indicate MDC-stained autophagosomes. B GFP-AtATG8e-labeled autophagosomes were visualized by fluorescence microscopy in 7-day-old GFP-AtATG8e and RNAi-2×GFP-AtATG8e seedlings. Arrows indicate GFP-labeled autophagosomes. Insets show an enlargement of the boxed areas. Scale bar = 50 µm for main figures, 10 µm for insets. C Seven-day-old GFP-AtATG8e and RNAi-2× GFP-AtATG8e seedlings were transferred to liquid MS medium containing 1 µM concanamycin A (+concA) or DMSO (-concA) as a solvent control for 12 h, followed by both fluorescence and DIC microscopy. Scale bar = 50 µm.