A Subset of Osteoblasts Expressing High Endogenous Levels of PPARγ Switches Fate to Adipocytes in the Rat Calvaria Cell Culture Model
Figure 4
Subcellular distribution of mesenchymal lineage determinants in RC cells.
Cells were cultured without BRL as described in Fig. 1. Proliferating cells (day3) (A), differentiating/nodule-forming cells (day 12) (B), and subcultures of differentiating/nodule-forming cells, i.e., cells from ALP+ fractions in developing RC cells (day 10) (C) were subjected to immunofluorescence staining for Runx2, PPARγ, Sox9, and MyoD. Left-side panels of A and B show proliferating and nodule-forming cells, respectively, by phase-contrast microscopy.