Inhibition of Iron Uptake Is Responsible for Differential Sensitivity to V-ATPase Inhibitors in Several Cancer Cell Lines
Figure 5
Bafilomycin induces apoptosis.
HeLa and SK-Mel-5 cells were treated with indicated concentrations of Baf for 24 hrs and then prepared for immunoblotting with antibodies to cleaved PARP or cleaved caspase 3. The cleaved proteins appear at much lower concentrations of Baf in SK-Mel-5 cells compared to HeLa, with the concentration difference between the appearance of cleaved protein similar to the differences in lethal doses for the two cell lines. The immunoblots shown are representative of two independent experiments.