A Clathrin Independent Macropinocytosis-Like Entry Mechanism Used by Bluetongue Virus-1 during Infection of BHK Cells
Figure 7
BTV-1 is co-localised with dextran during cell-entry.
BTV-1 was pre-bound to BHK cells and then internalised in the presence of Alexa-568 dextran (red) for 0.5 h. The cells were then processed for confocal microscopy using PM10 to detect input virus (green). Panels (A) and (B) shows dextran uptake for representative cells. Panels (C) and (D) shows virus uptake for the same cells as in panels A and B respectively. Panels (E) and (F) show merged images for the same cells. Yellow indicates co-localisation. The cell nuclei are shown in blue. Scale bar = 10 µm.