Downregulation of Homologous Recombination DNA Repair Genes by HDAC Inhibition in Prostate Cancer Is Mediated through the E2F1 Transcription Factor
Figure 2
Sensitivity of DU-145 cells to DNA damaging agents upon VPA treatment.
a) Clonogenic assay performed in DU-145 after treatment with different doses of VPA for 48 h before irradiation with different doses of radiation. Top panel shows representative clonogenic plates with 0Gy and 4 Gy radiation graph below depicts surviving fraction after VPA treatments and irradiation. Error bar represents standard deviation of three independent experiments. b) Clonogenic assay performed on DU-145 cells treated with 1.5 mM VPA and cisplatin (100 nM and 250 nM) for 48 h. Error bar represents standard deviation. c) Clonogenic assay performed on DU-145 cells treated with 1.5 mM VPA and hydroxyurea (0.5 mM and 1 mM) for 48 h. Error bar represents standard deviation.