Dissociation of CAK from Core TFIIH Reveals a Functional Link between XP-G/CS and the TFIIH Disassembly State
Figure 3
Dissociation of CAK complex from core TFIIH disrupts TFIIH integrity in XPG-deficient cells.
(A) Tight association between core TFIIH and CAK in NHF. (B–D) Dissociation of CAK from core TFIIH occurs in XPCS1LV, XPCS2LV and XP3BR cells with or without UV irradiation. (E) Restoration of TFIIH integrity in XPG cDNA-corrected XP3BR cells. (F) XPD is present in core TFIIH in XPG-deficient cells regardless of UV irradiation. Whole cell extracts were made from NHF and XPG-deficient cells and IP was performed as described for Figure 1B.