Structure-Function Relations in Oxaloacetate Decarboxylase Complex. Fluorescence and Infrared Approaches to Monitor Oxomalonate and Na+ Binding Effect
Figure 4
Effects of oxomalonate binding on the tertiary structure of OAD α subunit.
REES variations observed on the nonbiotinylated (a, c) or biotinylated (b, d) α subunit in absence (a, b) or presence (c, d) of 10 mM oxomalonate. Each point is the average of at least three determinations. Samples were in 250 mM NaCl, 0.5% Tween 20, 0.05% Brij 58, 50 mM Tris-HCl, pH 7.4 buffer.