Multiple Phosphatidylinositol 3-Kinases Regulate Vaccinia Virus Morphogenesis
Figure 2
PI3K inhibitors reduce late viral protein expression.
BHK cells were infected with VV at an MOI of 5, and PI3K inhibitors were added following entry. After 16 hours proteins were subjected to western analysis with various antibodies, including anti-Tubulin to ensure equal loading. Quantitation (right) is described in Methods. Data are expressed as the fold change relative to DMSO-treated samples. A. Cells were infected with VV strain WR expressing B5-GFP under its own promoter, and subjected to western analysis with GFP (top) or tubulin (bottom) antibodies. B. Cells were infected with VV strain WR expressing F13-GFP, and analyzed as in A. C. Cells were infected with VV strain WR for 6 or 16 hours. Filters were probed with P4b/4b or tubulin antibodies. Results are from two or three independent trials.