MicroRNA 128a Increases Intracellular ROS Level by Targeting Bmi-1 and Inhibits Medulloblastoma Cancer Cell Growth by Promoting Senescence
Figure 6
MiR-128a increases intracellular ROS by targeting Bmi-1.
A) Increased superoxide radical formation in Daoy cells that were transfected with miR-128a as detected using EPR spectra of the DMPO-OH spin adduct (closed circles in (ii)). The spectra collected are a signal average of 15 scans. The 1∶2∶2∶1(closed circles) quartet signal seen is due to the formation of DMPO-OH. The cells treated with miR-128a have ∼3 fold increase in ROS signal intensity (ii) as compared to the empty vector (i). Co-transfection of cells with miR-128a and Bmi-1 resulted in a decreased ROS level compared to that of miR-128a alone (iii). The treatment of SOD in miR-128a-transfected cells abolished the signals showing that the radical formed is mainly superoxide (iv). B) The quantitative analysis of EPR peak height normalized to cell number. *p<0.05 miR128a vs. pVETL and miR-128a + SOD.