Role of PAF Receptor in Proinflammatory Cytokine Expression in the Dorsal Root Ganglion and Tactile Allodynia in a Rodent Model of Neuropathic Pain
Figure 8
Upregulation of TNFα and IL-1β gene expression in the DRG is reduced in pafr−/− mice.
A, B. Real-time PCR analysis demonstrated that following nerve injury, the expression of TNFα (A) and IL-1β (B) mRNAs in the ipsilateral DRG of wild-type mice was much higher than in the contralateral side on day 7. However, pafr−/− mice failed to show upregulation of these cytokines and there were significant differences in these expressions of the ipsilateral side between wild-type and pafr−/− mice. Each measurement was normalized to 18S mRNA content. **p<0.01, ***p<0.001 compared with the contralateral side of wild-type mice. #p<0.05, ##p<0.01 compared with the ipsilateral side. n.s. means “not significant”. All data are presented as mean ± SEM of six to eight animals.