IsiA Is Required for the Formation of Photosystem I Supercomplexes and for Efficient State Transition in Synechocystis PCC 6803
Figure 3
Blue Native and SDS-PAGE analyses of the sucrose-gradient fractions.
Sucrose-gradient fractions from Fig. 1 were mixed with 1/10 volume of 5% Serva blue G (100 mM BisTris-HCl, pH 7.0, 0.5 M 6-amino-n-caproic acid, 30% glycerol) and separated by Blue Native PAGE (A) Single bands of the BN-PAGE were excised, denatured with a 1.5 X SDS sample buffer for 30 min at room temperature, and separated by SDS-PAGE (B) F3up and F3dn, the upper and lower green bands of the FeS F3 fraction in A, respectively.