A Role of Canonical Transient Receptor Potential 5 Channel in Neuronal Differentiation from A2B5 Neural Progenitor Cells
Figure 1
Isolation of A2B5+ cells and formation of neurospheres in the proliferation condition.
(A): FACS confirmed a high purity of A2B5+ MACS separation. A2B5+cell group after MACS separation contained 90.21% of A2B5+ cells (right). The 1.47% population is the same cells without secondary antibody. (B): A2B5+ cells cultured in ITSFn formed numerous neurospheres at one week after. (C): Secondary neurospheres were derived from cultured A2B5+ cells, after monthly passages. (D): A2B5+ cells were plated onto fibronectin to record SOCE in proliferation medium, (E): One or no sphere was found in cultured A2B5- cells until 4 weeks (F): Neurospheres were A2B5+/Nestn+. (G and H): Cultured A2B5- population consists of already-differentiated neuron, astrocyte, and oligodendrocyte. Scale bars, 50 µm.