Optimized Expression of Full-Length IgG1 Antibody in a Common E. coli Strain
Figure 1
Construction of tet promoter bacterial IgG expression plasmid.
pASK-IBA2 plasmid using a tet promoter was used as the backbone expression vector. The appropriate restriction sites for cloning in of the light and variable heavy chains, leader sequences (OmpA, PelB) and the constant heavy chain sequence (CH) were added. Light (LC) and variable chains (VH) were cloned in as a complete construct together with the intercistronic ribosomal binding site (RBS) from the phage display vector or as separate constructs from the 4G2 mammalian IgG expression vector.