Topoisomerase IIα Binding Domains of Adenomatous Polyposis Coli Influence Cell Cycle Progression and Aneuploidy
Figure 4
Expression of M2- or M3-APC results in G2/M accumulation in HL60 but not HL60/MX2 cells.
Graph shows the G2/M population from HL-60 and HL-60/MX2 cells expressing EGFP-M2- or M3-APC for 48 hours. Parental HL60 cells exhibit a 2-fold increase in G2/M when expressing M2-APC and a 4-fold increase when expressing M3-APC. A student's t-test demonstrated that these increases in G2/M were significant for M2 (* p = 0.02) and M3 (** p = 0.03), compared to cells expressing only EGFP. HL60/MX2 cells exhibit a decrease in G2/M when expressing M2- or M3-APC. However, these differences were not significant: M2 (p = 0.06) and M3 (p = 0.48), compared to cells expressing only EGFP. For both cell lines, 10,000 EGFP-positive cells were analyzed from three independent experiments.