Differential Expression of the Circadian Clock in Maternal and Embryonic Tissues of Mice
Figure 6
Acute luciferase activity in embryonic and maternal liver.
Per2::luc mice were used to make protein extracts from maternal and embryonic liver samples every four hours for 24 hours, during E18-19. Luciferase activity was measured as described in Methods. Maternal tissues showed a distinct peak and trough in PER2 levels, at ZT20 and ZT4. In contrast, embryonic liver showed no significant differences during the 24-hour period. Symbols represent the mean ± standard error of the mean (SEM) of three embryos per time point and three technical replicates from a single mother.