Cardiac Deletion of Smyd2 Is Dispensable for Mouse Heart Development
Figure 1
Temporal expression patterns of Smyd-family members during vertebrate heart development.
(A) Real-time qPCR showing relative expression levels of Smyd-family members in mouse heart ventricles at different developmental stages (E12.5 to adult as indicated). Expression levels of Smyd-family members were normalized to GAPDH Ct values as housekeeping gene. qPCR analysis of Smyd-family members −1, −2 and −5 reveals a peak mRNA expression for Smyd1 and Smyd2 between postnatal day 1 to day 5 while Smyd5 mRNA shows highest expression levels during embryonic stages E14.5 to E18.5 ceasing after birth. (B) Western-blots of pooled E12.5, P1 or adult tissue extracts (70 µg) from rat and mouse heart ventricles were probed with anti-Smyd2 antibody showing maximal expression at postnatal day 1. Blots were re-probed with anti-GAPDH antibody for equal loading control. (C) Semiquantitative RT-PCR for Smyd1, −2 and −5 expression in neonatal (P4) mouse tissues. 18s rRNA expression was used as loading control.