Protective Effect of Human Amniotic Fluid Stem Cells in an Immunodeficient Mouse Model of Acute Tubular Necrosis
Figure 8
Immunomodulatory effects of hAFSC once injected into glycerol induced ATN-damaged kidneys.
Mouse Cytokines were analyzed among all the experimental groups to evaluate changes in the inflammatory milieu. A. Developed multiplex cytokine assay membranes for mouse with ATN, mouse with ATN and Injection of PBS and Mouse with ATN and injection of hAFSC, showing the markedly different expression patterns of cytokines at 14 days. B. Schematic outline representation of mouse cytokine expression over the 14 days among different groups: 1. Mice with ATN only. As a general trend of cytokine expression, in these animals the pro-Inflammatory cytokines increased or remained highly expressed and anti-inflammatory cytokines showed decreased or low expression; 2. Mice with ATN and injection of hAFSC. As a general trend of cytokine expression, it is evident that the anti inflammatory cytokines increased over the 14 day study period, while pro-Inflammatory cytokines decreased; 3. Mice with ATN and injection of PBS. As a general trend of cytokine expression, in these animals the pro-Inflammatory cytokines increased or remained highly expressed and anti-inflammatory cytokines showed decreased or low expression; 4. Mice without ATN and injection of hAFSC. As a general trend of cytokine expression, in these mice the anti-inflammatory cytokines are mildly increased over the 14 days, while pro-inflammatory cytokines are not expressed or show decreased expression over the 14 days.