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Overexpression of the Endoplasmic Reticulum Chaperone BiP3 Regulates XA21-Mediated Innate Immunity in Rice

Figure 2

Overexpression of BiP3 Compromises XA21-Mediated Resistance.

(A) Rice lines 14 days after inoculation with Xoo strain PXO99Az. From left to right: Kitaake (Kit), transgenic line (Nat XA21) carrying Xa21 driven from its native promoter, and transgenic lines carrying BiP3 ox (BiP3 ox/Nat XA21). (B) Lesion length measurements of Xoo strain PXO99Az inoculated plants, BiP3 ox/Nat XA21 (4A-10, 5A-6, 7B-10, and 8A-12), Kitaake (Kit), and Nat XA21 control lines over 12 days. Each data point represents the average and standard deviation of at least four samples. (C) Xoo strain PXO99Az populations were monitored over 12 days in BiP3 ox/Nat XA21 (4A-10, 5A-6, 7B-10, and 8A-12), Kit, and Nat XA21 rice lines. For each time point, bacterial populations were determined in three separate leaves for each genotype. Capped vertical bars represent standard deviation values (cfu/leaf) obtained from the three samples.

Figure 2

doi: https://doi.org/10.1371/journal.pone.0009262.g002