Establishment of a Mouse Model with Misregulated Chromosome Condensation due to Defective Mcph1 Function
Figure 6
DNA damage response in Mcph1gt/gt cell culture.
(A) 53BP1 (green) localizes to repair foci in Mcph1gt/gt cells induced by treatment with the radiomimetic drug adriamycin. The foci co-localize with γ-H2AX foci (red) confirming that the focal staining represents indeed repair foci at the sites of DNA double strand breaks. (B) Overview pictures of 53BP1 foci formation (red) in wild type and Mcph1gt/gt cells following the induction of DNA double strand breaks by the radiomimetic drug bleomycin and treatment with Mcph1-siRNAs (bottom) and mock treatment (top). No obvious difference is detectable in the Mcph1gt/gt cells or siRNA treated cells compared to the controls. (C) Quantitative analyses of the same experiments. No significant difference in the formation of 53BP1 foci in the Mcph1gt/gt cells or siRNA treated cells compared to the controls could be detected. (D) Chromosomal breakage rates in Mcph1gt/gt and wt cells following ionizing irradiation with 2 Gy. There is no significant difference between wild type and Mcph1gt/gt cells.