Agroinfiltration Reduces ABA Levels and Suppresses Pseudomonas syringae-Elicited Salicylic Acid Production in Nicotiana tabacum
Figure 3
Heat-killed A. tumefaciens suppresses P. syringae-elicited SA production to a lesser extent than live A. tumefaciens.
A. The leaf shown was inoculated with A. tumefaciens GV3101 (AtGV3101 107 cfu/ml), heat-killed AtGV3101 (HK_At) or 10 mM MgCl2(AS) (Mg), followed by inoculation with P. syringae pv. tabaci 11528 (Pta), P. s. pv. tomato DC3000 (Pto) (105 cfu/ml) or 10 mM MgCl2 after 48 hours. The SA biosensor ADPWH-lux was inoculated into leaves 24 hours after infiltration with P. syringae. SA-induced luminescence was measured one hour after biosensor inoculation using a photon-counting camera. B. Absolute lux values were normalized against the infiltrated area. The bars show the average normalized luminescence values from three leaves. Error bars show standard deviation. General Linear Model (GLM) analysis revealed statistical differences between treatments (F = 81.2673; p<0.0001; df = 26). Means with the same letter were not significantly different at the 5% confidence level based on Tukey's HSD Test.