Dynamic Innate Immune Responses of Human Bronchial Epithelial Cells to Severe Acute Respiratory Syndrome-Associated Coronavirus Infection
Figure 8
Correlation between transcript and protein levels of genes encoding chemokines, cytokines and IFNs.
The relative transcriptional-versus-translational efficacies of selected genes in 2B4 cells were assessed, based on the fold-increase of mRNA and the corresponding protein levels of infected cells over those of mock-infected ones. A strong correlation between transcriptional and translational expressions of genes coding for CCL5/RANTES, CXCL1/GROα, CXCL10/IP-10, IFN-λ2, IL-6, IL-8, and TNFSF10/TRAIL (r2 = 0.8378) was identified in SARS-CoV-infected 2B4 cells. Dotted line indicates regression line (A). The relative efficacy of gene expression among those highly activated in SARS-CoV-infected and DHOV-infected 2B4 cells was compared as described in a subsection of Results. Dotted line (y = x) separates the genes to verify which virus (DOHV; upper part, SARS-CoV; lower part) -infected 2B4 cells effectively translate the genes than the other virus-infected one. It became clear that translational expressions of CCL5/RANTES, CXCL1/GROα, IL-1α, IL-6, and, particularly of IFN-β, IFN-λ1 and –λ2 were efficient in DHOV-infected 2B4 cells. The efficacy of CXCL-10/IP-10, IL-8 and TNFSF10/TRAIL expression was similar between these two differentially infected 2B4 cells (B). Results are shown as mean ± SEM for nine calculated results by division of each triplicated samples in indicated cytokines.