The E3 Ubiquitin-Ligase Bmi1/Ring1A Controls the Proteasomal Degradation of Top2α Cleavage Complex – A Potentially New Drug Target
Figure 3
Increased toxicity of VM26 following siRNA mediated reduction of Bmi1.
HeLa, A549 and MDA-MB-231 cells were transfected with Control (SC) or Bmi1 (X63) siRNAs. Following transfection, the sensitivity of the cells to different concentrations of VM26 was determined as described in material and methods. The LD50 of VM26 was calculated using Prism software (see text). The reduction of Bmi1 protein level by the siRNA is presented in the insert.